File:PCR.svg

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I am declaring this to be CC0. Although you are not legally required to do so, I would appreciate if you credit me (Madeleine Price Ball) when using my work. --Madeleine (talk) 02:20, 23 January 2013 (UTC)

Description
Nederlands: Nederlands: Schematische voorstelling van de PCR ketingreactie.
  1. De denaturatie van de de dubbele helix naar een enkele DNA-streng bij 96 °C.
  2. hybridizatie van de enkelstreng met primers bij 68 °C.
  3. vervollediging van de DNA-enkelstrengen bij 72 °C.
  4. Eind van de eerste cyclus.
in de praktijk wordt deze cyclus vele malen herhaald.


English: Diagram of PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers.


Schematic drawing of the PCR cycle.

  1. Denaturing at 96°C.
  2. Annealing at 68°C.
  3. Elongation at 72°C.
  4. The first cycle is complete. The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle.


Deutsch: Schematische Darstellung des PCR-Zyklus.
  1. Denaturalisieren/Schmelzen bei 96 °C.
  2. Anlagerung bei 68 °C.
  3. Verlängerung bei 72 °C.
  4. Der erste Zyklus ist beendet.


Čeština: Schéma PCR reakce demonstrující, jak amplifikace vede k exponenciálnímu růstu krátkého produktu ohraničeného primery.


Schematický nákres cyklu PCR.

  1. Denaturace při 96 °C.
  2. Nasednutí primerů při 68 °C.
  3. Syntéza DNA (prodloužení) při 72 °C.
  4. První cyklus je dokončen. Dvě výsledné řetězce DNA tvoří vzorovou DNA pro další cyklus, čímž se zdvojnásobí množství DNA duplikované v každém novém cyklu.
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