File:Neurite growth in a microfluidic device.webm

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Summary

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English: Neurons were cultured in a microfluidic channel (left) which was connected to a second channel (right) by narrow tunnels. The cell bodies in the left compartment were larger than the tunnels, but neurites are much smaller. The neurons extended neurites through the ~250 µm-long tunnels in less than 24 h. Here, the cells are dorsal root ganglion neurons. The microfluidic device is made of PDMS using soft lithography, then bonded to a glass substrate. The cells were adherent on the glass substrate. The time-lapse video over 48 hours was captured using differential interference contrast microscopy. Time is indicated as days:hours.
Date
Source

https://www.biorxiv.org/content/10.1101/2023.11.06.565744v2

https://doi.org/10.1039/D3LC00963G
Author Peter D. Jones, Beatriz Molina-Martínez, Anita Niedworok and Paolo Cesare

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Captions

Dorsal root ganglion neurons extend neurites in vitro (time lapse over 48 hours)

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6 November 2023

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