File:Site-directed mutagenesis library cloning steps.pdf
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Summary
| Description |
English: Depiction of one common way to clone a site-directed mutagenesis library (i.e., using degenerate oligos). The gene of interest is PCRed with oligos that contain a region that is perfectly complementary to the template (blue), and one that differs from the template by one or more nucleotides (red). Many such primers containing degeneracy in the non-complementary region are pooled into the same PCR, resulting in many different PCR products with different mutations in that region (individual mutants shown with different colors below). |
| Date | |
| Source | Own work |
| Author | Rob Hurt |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 21:57, 6 April 2020 |  | (394 KB) | wikimediacommons>Rob Hurt | Uploaded own work with UploadWizard |
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